Recombinant Human Cyclin B1 Protein

重组人Cyclin B1蛋白 | 重组人细胞周期蛋白B1

CCNB | CCNB 1 | ccnb1 | CCNB1_HUMAN | Cyclin B1 | G2 mitotic specific cyclin B1 | G2/mitotic-specific cyclin-B1

rp192056-10μg

10μg

无载体

≥90%(SDS-PAGE)

CCNB1, also known as cyclin B1, is a regulatory protein involved in mitosis. This protein complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Recombinant human CCNB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Post-translational: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132).Deubiquitinated by USP22 during G2/M phase (PubMed:27030811). Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

重组表达

49.2 kDa

N-His

53.7 & 144.6 kDa, under reducing conditions

E. coli Accession #: P14635 | E. coli

人(Human)

2-433 aa

MHHHHHHALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV

No

Yes