Recombinant Human ECHS1 Protein

重组人ECHS1蛋白 | 重组人烯脂酰辅酶A水合酶1蛋白

C80529 | EC 4.2.1.17 | ECHS 1 | Enoyl CoA hydratase 1 | Enoyl CoA hydratase mitochondrial | Enoyl CoA hydratase short chain 1 mitochondrial | Enoyl Coenzyme A hydratase short chain 1 | Enoyl Coenzyme A hydratase short chain 1 mitochondrial | Mitochondrial

rp192063-10μg

10μg

无载体

≥90%(SDS-PAGE)

Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:25125611, PubMed:26251176). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:26251176). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA) (PubMed:26251176). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (PubMed:26251176). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:25125611, PubMed:26251176). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity).

Testing in progress

重组表达

29.3 kDa

N-His

27.0 kDa, under reducing conditions; 27.0 kDa, under non-reducing conditions.

E. coli Accession #: P30084 | E. coli

<1.0 EU/μg

人(Human)

28-290 aa

MHHHHHHASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

No

Yes