Recombinant Human BTK Protein

重组人BTK蛋白 | 重组人Bruton酪氨酸激酶蛋白 | 重组人布鲁顿酪氨酸激酶蛋白

Agammaglobulinaemia tyrosine kinase | AGMX 1 | AGMX1 | AT | ATK | B cell progenitor kinase | B-cell progenitor kinase | BPK | Bruton agammaglobulinemia tyrosine kinase | Bruton tyrosine kinase | Bruton’s Tyrosine Kinase | Btk | BTK_HUMAN | dominant-negati

rp192263-10μg

10μg

无载体

≥95%(SDS-PAGE)

Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation. Post-translational: Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.

重组表达

12.1 kDa

N-His

11.4 kDa, under reducing conditions; 11.8 & 25.2 kDa, under non-reducing conditions.

E. coli Accession #: Q06187 | E. coli

<1.0 EU/μg

人(Human)

280-377 aa

MhhhhhhEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPV​

No

Yes