重组耐碱蛋白A

重组蛋白A | 重组蛋白A | 重组葡萄球菌蛋白A

Protein A | Staphylococcal Protein A | Staphylococcal Protein A Recombinant | Immunoglobulin G-binding protein A | gG-binding protein A | Immunoglobulin G binding protein A | SPA | rat-PA

rp192268-10mg

10mg

无动物源

≥90%(HPLC)

本品为大肠杆菌重组表达的耐碱蛋白A，经层析纯化、冷冻干燥得到的重组蛋白A冻干粉。阿拉丁重组蛋白A是在天然金黄色葡萄球菌蛋白A的基础上对蛋白质结构进行改造，由4个Fc结合结构域片段串联而成，分子量27KD，与天然蛋白A相比，去除了与免疫球蛋白Fab区的结合位点，增强了其与Fc区的结合能力，同时提高了蛋白质的稳定性和耐碱性。重组蛋白A与琼脂糖凝胶键合后可制备用于抗体纯化的亲和填料，可从腹水、血清、细胞培养上清或细胞抽提物中分离和纯化多种哺乳动物不同亚型的抗体或包含抗体Fc片段的基因工程重组蛋白。阿拉丁重组耐碱蛋白A的C端含有一个半胱氨酸，可增强其同凝胶的定向共价键合。 2、用途 生化研究：用于免疫分析和IgG的提纯。 制备用于抗体纯化的亲和填料：可从腹水、血清、细胞培养上清或细胞抽提物中分离和纯化多种哺乳动物丌同亚型的抗体或包含抗体Fc片段的基因工程重组蛋白。 3、推荐使用方法 本品为大肠杆菌重组表达的耐碱蛋白A，其C末端设计有半胱氨酸残基，可以通过其上的巯基不环氧基团进行偶联反应，制备亲和层析填料。基球环氧活化以及蛋白A偶联方法需经实验后确定。 This product is a freeze-dried powder of recombinant alkali resistant protein A expressed by Escherichia coli and purified by chromatography and freeze-drying. Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can be bonded to agarose gel to prepare an affinity filler for antibody purification. It can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts. The C-terminal of Aladdin recombinant alkali resistant protein A contains a cysteine, which can enhance its directional covalent bonding with gel. 2、Purpose Biochemical research: used for immune analysis and purification of IgG. Preparation of affinity fillers for antibody purification: Multiple mammalian subtypes of antibodies or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant, or cell extracts. 3、Recommended usage This product is a recombinant expression of alkali resistant protein A in Escherichia coli. Its C-terminus is designed with a cysteine residue, which can be coupled through the thiol non epoxy group on it to prepare affinity chromatography fillers. The activation of epoxy groups and protein A coupling methods need to be determined through experiments.

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

重组表达

27 kDa

No tag

24.3-29.7 kDa, under reducing conditions

E. coli Accession #: P02976 | E. coli

<5.0 EU/mg

金黄色葡萄球菌(Staphylococcus aureus)

Yes

Yes