Recombinant Protein L

重组蛋白L | 重组大消化链球菌蛋白L | 重组蛋白L

ProteinL | RPL | Recombinant Peptostreptococcus magnus Protein L | Peptostreptococcus magnus Protein L | Protein L

rp192270-1mg

1mg

无动物源

≥90%(SDS-PAGE)

aladdin生产的重组蛋白L（Protein L）由原核系统表达、经分离纯化、除菌过滤等步骤制成。能够通过kappa链和多种免疫球蛋白相结合，分子量为35.4 kDa。与蛋白A和蛋白G相比，蛋白L能更广泛地结合各种来源及亚类的抗体，包括人、小鼠、大鼠、兔和鸡，但不结合牛、犬、山羊或绵羊来源的免疫球蛋白。重组蛋白L可以广泛应用于制备抗体亲和层析柱、亲和磁珠和ELISA试验等，常用于抗体或抗体片段的纯化、指示物的标记（如荧光、酶、胶体金、生物素）等。 The recombinant protein L produced by aladdin is expressed in a prokaryotic system, purified, sterilized, and filtered. It can bind to various immunoglobulins through kappa chains, with a molecular weight of 35.4 kDa. Compared with protein A and protein G, protein L can bind to antibodies from various sources and subtypes more widely, including humans, mice, rats, rabbits, and chickens, but does not bind to immunoglobulin from cattle, dogs, goats, or sheep sources. Recombinant protein L can be widely used in the preparation of antibody affinity chromatography columns, affinity magnetic beads, ELISA assays, etc. It is commonly used for purification of antibodies or antibody fragments, labeling of indicators (such as fluorescence, enzymes, colloidal gold, biotin), etc.

Protein L is a 36,000 dalton immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains - about 65% of human immunoglobulins carry kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

重组表达

35.4 kDa

N-His

35.4 kDa, under reducing conditions

E. coli Accession #: D6S9W1 | E. coli

大白金菊/大白粉蝶(Finegoldia magna/Peptostreptoccus magnus)

Yes

Yes