彩色预染蛋白分子量标准 (25-300kDa)

三色预染蛋白Marker | 多色低分子量蛋白分子量标准 | 彩色预染蛋白分子量标准 | 蛋白Ladder

Prestained Color Protein Marker | Prestained Color Protein Ladder | Broad Multi Color Pre-Stained Protein Standard | Star Ribbon Pre-stained Protein Marker | Tricolor Pre-Stained Protein Marker | Protein Ladder

rp192284-4×250μl

4×250μl

25-300kDa

25 Green/ 45 Blue/ 72 Orange/ 100 Blue/ 130 Blue/ 160 Blue/ 200 Blue/ 250 Blue/ 300 Blue kDa

彩色预染蛋白分子量标准： 1. 该产品包含了从25kDa到300kDa共9种高度纯化并预染的重组蛋白质。 2. 适合作为SDS-PAGE和Western的蛋白质分子量标准。 3. 本彩色预染蛋白质分子量标准已经配制在1×SDS-PAGE上样缓冲液中，直接使用，不要煮沸、稀释和加入还原剂处理。 4. 根据上样孔的大小，本彩色预染蛋白质分子量标准通常每次上样10微升（5×1.5mm胶孔10ul足够），即可在电泳时、电泳后和转膜后观察到非常清楚的蛋白条带。 预染蛋白质分子量标准经非预染蛋白质分子量标准标定，具有如下突出优势： 1. 蓝色和绿色条带在Tris-Glycine，Bis-Tris，Tris-Acetate缓冲体系SDS-PAGE胶其表观分子量基本没有变化。 2. 橙(红)色条带与进口产品变化一致。 使用方法： 1. 室温下解冻后完全溶解并轻轻充分混匀，不要煮沸。 2. 取本产品10µl与实验样品同时进行聚丙烯酰胺凝胶电泳；建议有条件的实验室在初次使用本产品时可以根据自身的实验条件和实验习惯通过预实验确定合适的上样量，这样可以节约成本，同时获得效果更佳的实验图片。 3. 未使用的彩色预染蛋白分子量标准保存于储存条件，在4°C可放置2个月。 注意事项： 1. 注意电泳时间，低分子量蛋白可能会泳动出蛋白胶前沿。 2. Westernblot时需要注意小分子特殊性，特别对于小于3kDa的条带，容易穿膜而过。另外建议转膜液不添加SDS，若实验必须使用，使用SDS浓度不超过 0.02%-0.04%。 3. 预染蛋白质在不同的缓冲体系下有不同的表观分子量，如果在该缓冲体系中事先用非预染蛋白质标定，可以大致确定蛋白质分子量。 Color pre dyed protein molecular weight standard: 1. This product contains 9 highly purified and pre stained recombinant proteins ranging from 25kDa to 300kDa. 2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting. 3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents. 4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 10 microliters of sample per loading (10μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer. The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages: 1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems. 2. The orange (red) color stripes are consistent with the changes in imported products. Usage method: 1. After thawing at room temperature, completely dissolve and gently mix well, do not boil. 2. Take 10µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images. 3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months. Matters needing attention: 1. Pay attention to the electrophoresis time, as low molecular weight proteins may swim out of the protein gel front. 2. When Western blotting, attention should be paid to the specificity of small molecules, especially for bands smaller than 3kDa, which are prone to pass through the membrane. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, the concentration of SDS should not exceed 0.02% -0.04%. 3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.

25 Green/ 45 Blue/ 72 Orange/ 100 Blue/ 130 Blue/ 160 Blue/ 200 Blue/ 250 Blue/ 300 Blue kDa