Recombinant Human Lck Protein

重组人Lck蛋白 | 重组人淋巴细胞特异性酪氨酸蛋白激酶Lck

EC 2.7.10 | EC 2.7.10.2 | Hck-3 | Lck | Lcktkr | Leukocyte C-terminal Src kinase | LSK | LSTRA | Lymphocyte cell-specific protein-tyrosine kinase | lymphocyte-specific protein tyrosine kinase | p56(LSTRA) protein-tyrosine kinase | p56lck | p56-LCK | pp58l

rp214492-10μg

10μg

无载体

≥90%(SDS-PAGE)

Lck (p56lck) is a member of the Src family of non-receptor tyrosine kinases. It was identified as a gene rearranged and over-expressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene. Lck behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show over-expression of Lck, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers. Post-translational: Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Dephosphorylation at Tyr-394 by DUSP22 negatively regulates T-cell receptor signaling (PubMed:24714587, PubMed:38225265). Myristoylation is required prior to palmitoylation.Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2.'Lys-63'-linked ubiquitinated at Lys-99 and Lys-276 by UBR2; this modification is required for autophosphorylation at Tyr-394.

重组表达

27.2 kDa

N-His

27.1 & 53.0 kDa, under reducing conditions; 25.7 & 27.0 & 49.9 & 51.8 kDa, under non-reducing conditions.

E. coli Accession #: P06239 | E. coli

<1.0 EU/μg

人(Human)

2-234aa

MHHHHHHGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWW

No

Yes