氨曲南酶

Aztreonam Enzyme

rp216621-3ml

3ml

BioReagent

氨曲南专用酶是采用重组基因的水溶性蛋白，呈深褐色液体。根据氨曲南的化学结构而构建的重组基因蛋白。其基因片断的活性中心，可破坏氨曲南的化学结构式，使开环/断链后失去该药物的 抗菌性。 做抗生素的无菌检查时，应用手工注射器吸取无菌水注入药物的西林瓶中，摇匀，溶解，再吸出。加入至0.9%氯化钠的500ml溶液中，摇匀，不可用培养器上的针头溶解，输入，避免高浓度的溶液 通过滤膜，造成难以冲洗彻底。 这里，再次强调：做抗生素的无菌检查时，一定要手工注射器 注入无菌水溶解样品，再把溶解的样品转移至0.9%氯化钠的500ml溶液中摇匀，使得样品不会造成局部浓度过高，通过滤膜时难以冲洗彻底的情况。 无菌检验时，取酶2ml加入无菌水3ml，摇匀，使成氨曲南 专用酶的稀释液，取2ml酶的稀释液放入1500ml的冲洗液摇匀。待冲洗液冲洗培养器的滤膜完成后，泵尽。用手工注射器在三个培养器的呼吸口刺入，各加1ml的稀释酶，尽量让酶平铺在滤膜整个面，静止10分钟，使高浓度的酶与培养器滤膜充分接触，以破坏（中和、灭活）滤膜上残留的氨曲南药物，然后再泵入相应的培养基，摇匀。阳性对照管加入1ml的对应试验菌。 在冲洗液加2ml的氨曲南专用酶的稀释液，可以去除滤膜内部 残留的氨曲南的少量药物抗菌性。 在三个培养器加1ml的氨曲南专用酶的稀释液，可以去除培养器内部壁上和滤膜表面残留氨曲南的少量药物抗菌性。 每天上午、下午各对阳性对照管轻轻摇匀一次。 客户可根据上述做方法学验证，也可根据实际操作情况做验证。 The special enzyme of amtronam is a water-soluble protein using recombinant gene, which is dark brown liquid. A recombinant gene protein constructed according to the chemical structure of amtronam. The active center of its gene fragment can destroy the chemical structural formula of amtronam, so that the antimicrobial properties of the drug are lost after ring-opening/chain breaking. When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly. Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane. During sterility test, add 2ml of enzyme into 3ml of sterile water and shake well to make diluent of special enzyme for amtronam. Add 2ml diluent of enzyme into 1500ml of rinsing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. A manual syringe was used to Pierce the respiratory mouth of the three incubators, and 1ml of diluent enzyme was added to each of the three incubators, and the enzyme was spread on the entire surface of the filter membrane as far as possible. Then, the high concentration of enzyme was fully in contact with the filter membrane of the incubator, so as to destroy (neutralize and inactivate) the residual amtraxam drug on the filter membrane, and then pumped into the corresponding medium and shook well. Positive pairs were treated with 1ml of corresponding test bacteria. Adding 2ml diluent of special enzyme of amtronam to the rinse solution can remove a small amount of antimicrobial properties of amtronam remaining in the filter membrane. Adding 1ml diluent of special enzyme for amtronam to three incubators can remove a small amount of antimicrobial activity of amtronam remaining on the inner wall of the incubators and on the surface of the filter membrane. Shake the positive pair gently once a day in the morning and afternoon. Customers can do methodological verification according to the above, but also according to the actual operation of the verification.