Recombinant Human FAK Protein

重组人 FAK 蛋白 | 重组人粘着斑激酶1蛋白

EC 2.7.10 | FADK 1 | FADK1 | FADKFAK-related non-kinase polypeptide | FAK | FAK1FRNK | FAKEC 2.7.10.2 | focal adhesion kinase 1 | pp125FAK | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | PTK2

rp217136-10μg

10μg

无载体

≥90%(SDS-PAGE)

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness. Elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples has been demonstrated. Post-translational: Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21. Phosphorylation on tyrosine residues is enhanced by NTN1 (By similarity). Sumoylated; this enhances autophosphorylation.

重组表达

27.9 kDa

N-His

27.5 kDa, under reducing conditions; 27.5 kDa, under non-reducing conditions.

E. coli Accession #: Q05397 | E. coli

人(Human)

798-1041 aa

MHHHHHHGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQA

No

Yes