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Product details

名称

Recombinant Human DNMT1 Protein

别名

重组人DNMT1蛋白 | 重组人DNA甲基转移酶1蛋白

英文别名

货号

rp217144-10μg

包装规格

10μg

级别

无载体

浓度

≥85%(SDS-PAGE)

生化机理

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Post-translational: Sumoylated; sumoylation increases activity.Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 thereby increasing DNMT1 stability.Methylation at Lys-142 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation.Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

来源

重组表达

预测分子量

24.3 kDa

蛋白标签

N-His

SDS-PAGE

26.8 kDa, under reducing conditions; 22.6 & 25.1 & 26.2 & 47.0 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P26358 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

1381-1587 aa

序列

MHHHHHHASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKA

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

user reviews

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