Share to

Details

Safety

Technical Documents

Articles

reviews

Consult

returns

Product details

名称

Recombinant Human RAF1 Protein

别名

重组人RAF1蛋白 | 重组人Raf-1原癌基因，丝氨酸/苏氨酸激酶蛋白

英文别名

cRaf | c-Raf | c-Raf-1 | CRAFraf proto-oncogene serine/threonine protein kinase | EC 2.7.11 | EC 2.7.11.1 | NS5 | Proto-oncogene c-RAF | RAF proto-oncogene serine/threonine-protein kinase | RAF | Raf1 | Raf-1 | Raf-1Oncogene RAF1 | v-raf-1 murine leukemia

货号

rp217603-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. Post-translational: Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35831509, PubMed:35830882). Phosphorylation at Ser-338 by PAK1 and PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization. Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the protein, possibly by preventing proteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338 and Ser-621 are somehow linked to the methylation potential of cells. Treatment of cells with HGF in the presence of the methylation inhibitor 5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and Ser-621 and decreased phosphorylation at Ser-296, Ser-301 and Ser-338. Dephosphorylation at Ser-338 by PPP5C results in an activity decrease. Methylated at Arg-563 in response to EGF treatment. This modification leads to destabilization of the protein, possibly through proteasomal degradation.

来源

重组表达

预测分子量

19.1 kDa

蛋白标签

N-His

SDS-PAGE

19.6 kDa, under reducing conditions; 19.6 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P04049 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

189-353 aa

序列

MHHHHHHNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLST

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

user reviews

Consultation

Warm reminder：

Because the manufacturer may temporarily adjust product specifications, prices, inventory, parameters and other information without notice, and there are differences in each user's consultation time and procurement needs, the platform reply content is only of reference value within a certain period. Sorry for the inconvenience caused to you, and thank you for your understanding and support! Please consult the online customer service for the latest details. Everything is subject to the real-time reply of the customer service.！

After Sales Services

Please check the quantity, appearance and product information in time when you receive the goods. In case of damage, wrong delivery, abnormal quality and other problems, please feel free to contact us.

Service Hotline:400-6226-992 ，We will handle it for you as soon as possible, thank you for your support and trust.！