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Product details
名称
重组胃蛋白酶(质谱级)
别名
胃蛋白酶 | 质谱级胃蛋白酶 | 测序级胃蛋白酶
英文别名
Pepsin | Pepsin (MS Grade) | Sequencing grade pepsin
货号
rp218612-250mg
包装规格
250mg
级别
无动物源
浓度
≥20000 U/g Protein
产品介绍
胃蛋白酶(Pepsin)优先在苯丙氨酸、亮氨酸、酪氨酸和色氨酸的C端进行水解。可用于血清制品中酶消化,酪蛋白消化,单独或与其他蛋白酶联合在质谱分析和其他应用中进行蛋白分析等。 推荐使用方法 1血清制品 (1)将待处理样品用酸调pH至所需要的工艺参数范围; (2)根据重组胃蛋白酶的活性及工艺需要调整酶的加入比例,例如3-12U/mL; (3)取样进行非还原性电泳分析酶切效果,或可采用其他需要的分析方法进行分析。 2蛋白分析 (1)配制目的蛋白溶液:将蛋白溶解于pH1.0-5.0缓冲液中,或将蛋白先溶解于pH7.5缓冲液中然后加入1MHCl,HCl终浓度约为40-50mM; (2)将胃蛋白酶加入目的蛋白溶液中,推荐比例为,酶:蛋白=1:20-1:100(W:W); (3)37°C反应1-18h; (4)95°C加热10min终止反应。 储存和运输稳定性 (1)储存稳定性:重组胃蛋白酶储存于2-8°C,2年稳定; (2)运输稳定性:冰袋运输,活性稳定。 产品优势 (1)纯度高:基因工程重组表达所得,纯度高,无杂酶污染。 (2)质量稳定:批量生产,可保证稳定连续的批次生产;产品批次间无差异,质量稳定。 (3)安全性高:重组生产,无外源性病毒污染,生产过程不使用任何动物源性原料。 产品用途 (1)血清制品中酶消化; (2)单独或与其他蛋白酶联合在质谱分析和其他应用中进行蛋白分析; (3)用于结构蛋白研究和抗体分析; (4)用于消化蛋白质组学上紧密折叠蛋白; (5)疫苗生产消化酪蛋白。
生化机理
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
生物活性
Activity: ≥10000 U/g Powder. Specific Activity (intemal): ≥20000 U/g Protein.
来源
重组表达
表达系统
酵母 Accession #: P00791 | 酵母
种属
Pig
无动物源
Yes
无载体
Yes
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
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「No technical documents」
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Research chemical 250mg

Research chemical 250mg; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 250mg.

item number:rp218612-250mg
Product model:250mg
level: 250mg
Lead Time:30days
sold 550 Items
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Specifications:

250mg

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