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Product details
名称
重组人α-糜蛋白酶(质谱级)
别名
胰凝乳蛋白酶 | α-糜蛋白酶 | α-胰凝乳蛋白酶 | 重组糜蛋白酶 | 重组胰凝乳蛋白酶
英文别名
Chymotrypsin Sequencing Grade | CTRB1 | CTRB | Chymotrypsinogen B | Recombinant Human Chymotrypsin‌
货号
rp218613-20μg
包装规格
20μg
级别
无动物源
浓度
≥90%(SDS-PAGE)
产品介绍
α-糜蛋白酶,又名α-胰凝乳蛋白酶,是一种丝氨酸蛋白酶,可以水解酪氨酸,色氨酸和苯丙氨酸的羧基端肽键。Ca2+是α-糜蛋白酶的激活剂和稳定剂,同时该酶被二异丙基氟磷酸盐(DFP),甲苯磺酰氟(PMSF),N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK),胰凝乳蛋白酶抑制剂,抑肽酶,α1-抗胰蛋白酶和α2-巨球蛋白,10mM Cu2+和Hg2+所抑制。阿拉丁的重组人α-糜蛋白酶采用毕赤酵母表达,经过多步纯化获得,没有其它蛋白酶污染。 酶活定义:在pH 7.8,25°C条件下,每分钟水解1.0 μmol BTEE的酶量定义为1U。 运输:冰袋或干冰保温运输,活性稳定。 产品应用: 肽图分析;蛋白质鉴定 使用方法(仅供参考) 1、建议使用前100-200g离心2分钟,然后小心开盖加入1mM HCl复溶,至终浓 1.0 mg/mL,然后盖上管盖上下颠倒或者振荡充分混匀。复溶后可在4°C或者-20°C保存至少3个月,复溶后尽量避免反复冻融。 2、本产品复溶后可以直接应用于肽图分析或者蛋白质鉴定,推荐的添加比例为1:20-1:200 (W:W),在25°C下酶解2-18h。 优势 (1)重组表达,无其它蛋白酶污染 (2)纯度高,可应用于蛋白质分析 注意事项 1、使用时请穿实验服并佩戴一次性手套。 2、本品不得直接用于临床诊断和治疗。
生物活性
≥70 U/mg powder
来源
重组表达
表达系统
毕赤酵母 Accession #: P17538 | 毕赤酵母
种属
人(Human)
无动物源
Yes
无载体
Yes
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
「No technical documents」
Articles
「No related articles yet」
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Research chemical ≥90%

Research chemical ≥90%; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: ≥90%.

item number:rp218613-20μg
Product model:20μg
level: ≥90%
Lead Time:30days
sold 567 Items
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