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名称
酯酶 来源于猪肝
别名
羧基酯水解酶 | 肝羧酸酯酶
英文别名
Carboxylesterase | carboxyl ester hydrolases | LOC100736962 | PLE-1 | PLE-G2
货号
rp221828-100KU
包装规格
100KU
级别
生物活性
浓度
≥15 U/mg powder
产品介绍
酯酶,又称羧基酯水解酶,是水解酶超家族中的一员。酯酶在生物体内广泛存在,如在消化系统中帮助消化食物中的酯类物质,同时也在细胞内参与脂肪酸代谢等生物过程。酯酶在工业上有广泛应用,如在生产生物柴油、食品添加剂和化妆品等行业中。来源于:猪肝。 产品特性 酯酶单体分子量约为 66kDa。它是一种丝氨酸酶,每个分子上有两个活性位点,它在稀酸或浓盐存在下解离成活性半分子。 稳定性 酯酶作用于含有短链脂肪酸的水溶性羧酸酯。其功能来自于 Ser、His 和 Asp/Glu 的催化三联体,在低温低 PH环境下三联体形式稳定。 用途和作用 1、用于有机化学中的动力学拆分和不对称合成。 2、用于揭示紫外可见光谱中的荧光。 3、用于制药和工业应用中以催化五乙酰儿茶素和表儿茶素的水解。 4、用于开发一种基于凝胶的替代实验方案,用于水性介质中的珠结合催化活性的筛选。 注意事项 1、避免与皮肤和眼睛接触。 2、为了您的安全和健康,请穿实验服并戴一次性手套操作。 Esterase, also known as carboxyl ester hydrolases, are a member of the hydrolytic enzyme superfamily. Esterase is widely present in organisms, such as helping to digest esters in food in the digestive system, and also participating in biological processes such as fatty acid metabolism within cells. Esterase is widely used in industry, such as in the production of biodiesel, food additives, and cosmetics. ≥15U /mg powder, ≥100 U/mg protein. Source: Porcine liver. Properties The molecular weight of esterase monomer is approximately 66kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half molecules in the presence of dilute acid or concentrated salt Stability Esterase acts on water-soluble carboxylic acid esters containing short chain fatty acids. Its function comes from the catalytic triad of Ser, His, and Asp/Glu, which is stable in the form of a triad under low temperature and low pH conditions. Application 1、Used for kinetic resolution and asymmetric synthesis in organic chemistry. 2、Used to reveal fluorescence in the UV visible spectrum. 3、Used in pharmaceutical and industrial applications to catalyze the hydrolysis of pentaacetyl catechins and epicatechin. 4、It is used to develop an alternative experimental scheme based on gel for screening bead binding catalytic activity in aqueous media. Attention 1、 Avoid contact with skin and eyes. 2、 For your safety and health, please wear Lab coat and disposable gloves.
生物活性
活性: ≥15 单位/毫克粉末。比活力:≥100 单位/毫克蛋白。单位定义: 一个酶活性单位是指在 pH 8.0、25°C条件下每分钟将1.0 μM 丁酸乙酯水解成丁酸和乙醇所需的酶量。
来源
天然
预测分子量
66 kDa
种属
猪(Porcine)
Safety
「No Dangerous Attributes」
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Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:rp221828-100KU
Product model:100KU
level: biological activity
Lead Time:30days
sold 679 Items
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