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Product details
名称
重组糖苷内切酶S2 (Endo S2)
别名
内切糖苷酶S2
英文别名
Endoglycosidase S2 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase | di-N-acetylchitobiosyl beta-N-acetylglucosaminidase | endo-beta-N-acetylglucosaminidase | endoS2
货号
rp223111-2KU
包装规格
2KU
级别
生物活性
浓度
≥90%(SDS-PAGE)
产品介绍
Endo S2 全称内切糖苷酶 S2 ,基因来源于酿脓链球菌(Streptococcus pyogenes),是一种高度特异性的内切糖苷酶,可以从 IgG 上切除具有或没有核心岩藻糖基化的高甘露糖型、杂合型、双天线复杂型 N-聚糖,还可以将噁唑啉形式的 N-聚糖转移到经 Endo S2 水解后的有或者没有核心岩藻糖的抗体 N-糖基化位点。本产品活性高,稳定性好,可以用于抗体糖基化修饰和分析。 反应式 使用方法 1、用去离子水溶解 1-20 µg 的糖蛋白,加入1 µl 的 500 mM Tris-HCl(pH7.5),用去离子水定容到 10 µl; 2、加入1-2 µl 的 Endo S2,轻轻吹打混匀; 3、37℃孵育 60-120 min; 4、用于 SDS-PAGE 分析或质谱分析。 【注】多数常见的生物缓冲液适用,比如 Tris 或 PBS,不在此 pH 范围的缓冲液(如醋酸缓冲液)可能 适用,但是孵育时间或酶量需要根据实际情况进行优化。 注意事项 为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
生物活性
≥20 KU/mL,单位定义:10 μl的反应体系中,37 °C 反应1小时从5 μg 人 IgG 中除去超过 95%的碳水化合物所需要的酶量。
来源
重组表达
蛋白标签
C-His
SDS-PAGE
92kDa
表达系统
E. coli Accession #: T1WGN1 | E. coli
种属
酿脓链球菌(Streptococcus pyogenes)
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
「No technical documents」
Articles
「No related articles yet」
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Research chemical ≥90%

Research chemical ≥90%; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: ≥90%.

item number:rp223111-2KU
Product model:2KU
level: ≥90%
Lead Time:30days
sold 989 Items
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