N-糖苷酶F（质谱级）

PNGase F（质谱级） | 糖基肽酶F | 糖苷酶F 来源于脑膜炎脓毒性黄杆菌 | 糖苷酶F 来源于脑膜脓毒性金黄杆菌 | 肽N-糖苷酶 | N-糖酰胺水解酶F

N-Glycosidase F | Peptide N-glycosidase | peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase | glycopeptidase | glycopeptide N-glycosidase | N-glycanase | N-oligosaccharide glycopeptidase | ngl | PeptideN-Glycosidase F

rp227283-15KU

15KU

无动物源

≥95%(SDS-PAGE)

PNGase F全称Peptide N-Glycosidase F，也称N-糖酰胺水解酶F，是一种来源于Elizabethkingia miricola (formerly Flavobacterium meningosepticum) ，通过E.coli重组表达的糖基肽酶。PNGase F可以在几乎所有类型的N-多糖如高甘露糖、混合型和复杂型寡聚糖的最内端N-乙酰葡萄糖胺 (GlcNAc)和天冬酰胺残基的连接处切割。 产品用途 适用于抗体、免疫球蛋白融合蛋白或其它糖蛋白体外去除N-糖基化和结构表征。 产品特点 高纯度： 没有污染蛋白酶或其它糖苷酶，纯度≥95％。 质量稳定： 严格质控，每批产品均经过活性和功能验证，保证产品质量。 产品原理 组分表 rp227283

Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain. PNGase F binds primarily to the polypeptide chain around the glycosamine junction including the inner di-N-acetylchitobiose core on the carbohydrate moiety. Catalytic activity: Hydrolysis of an N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.

≥100000 U/mL

E. coli Accession #: P21163