What is required to grow bacteria? How to simplify
bacteriaCultivation is a technique for the growth and reproduction of bacteria by artificial methods. Bacteria are widely distributed in nature, with a large number and variety. They can benefit humans and become a cause of disease. Most bacteria can be cultured artificially by inoculating them inmediumCultured bacteria are used for research, identification and application. Bacterial culture is a complex technique.
Due to the ubiquity of bacteria, the entire culture process must be carried out according to the requirements of aseptic operation from the time of preparation of the culture medium, otherwise the contamination of the specimen by external bacteria will lead to wrong results; and once the cultured pathogenic bacteria contaminate the environment, it will cause cross-infection.
For cultures conducted for the purpose of disease diagnosis, appropriate specimens (blood, urine, stool, pus, secretions, etc.) should be selected, and the results should be interpreted in light of the clinical situation.
Take the photosynthetic bacteria culture method as an example. The method of photosynthetic bacteria culture is divided into four steps in sequence: disinfection of containers and tools, preparation of culture medium, inoculation and culture management.
(1) Disinfection of containers and tools.
(Ii) Preparation of culture medium
(3) After the inoculation medium is prepared, the inoculation should be carried out immediately. The amount of photosynthetic bacteria produced in culture is relatively high, generally 20% to 50%, that is, although the ratio of the mother liquor and the newly formulated medium is 1:4 to 1:1, it should not be less than 20%, especially for micro-gas culture, the inoculation amount should be higher, otherwise it is difficult for photosynthetic bacteria to dominate in the culture medium, affecting the final yield and quality of the culture.
(IV) Cultivation and management During the cultivation process of photosynthetic bacteria, management work includes three aspects: daily management operations and testing, observation and inspection of growth conditions, and analysis and handling of problems.
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