Taq SYBR® Green qPCR Premix (Universal)

2026-03-27 10:24:45

Taq SYBR® Green qPCR Premix (Universal)

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brand

Annoron-Annoron

item number

AS050038

Product classification

PCR series

Research Area

Taq SYBR® Green qPCR Premix

(Universal)

REF: AS050038

Storage and transportation conditions

For long-term storage, please refer to -20 ℃ Store in the dark, Mix After melting, it can be found in Store stably at 4 ° C in the dark for one month, and try to avoid repeated freezing and thawing.

Product composition

component	Specifications
Taq SYBR® Green qPCR Premix (Universal)	5× 1 ml

Product introduction

Taq SYBR® Green qPCR Premix Yes SYBR ® Green I chimeric dye method dedicated qPCR reagent, a 2x premix containing all ingredients other than primers and DNA samples Yes. qPCR components can reduce the operation steps, shorten the sampling time, and reduce the probability of contamination. its The core component is an antibody-modified hot start. Taq DNA Polymerase with Optimized Buffers System and PCR reaction promotion factor makes the product have the characteristics of strong specificity and high amplification efficiency, effectively inhibits non-specific amplification, and can accurately quantify templates with a wide concentration range. Obtain stable and reliable qPCR results.

The premix contains unique correction dyes that are compatible with a range of qPCR devices, including those that require ROX correction. No additional dyes are required to correct the instrument during experimental operation.

How to use

1. Use attention

① because Mixing is premixed with dyes, and the preservation or preparation of the reaction system should avoid strong light exposure. Gently mix upside down before use Mix, do not vortex oscillation and mix well to avoid excessive production bubble ;

③ Mix It contains Universal calibrated dyes, available on all models, no additional dyes required.

2. suggested qPCR reaction system

reagent	usage	final concentration
Taq SYBR® Green qPCR Premix	10 m	1×
forward primer (10 (μM)a	0.4 m	0.2 mM
reverse primer (10 (μM)a	0.4 m	0.2 mM
DNA template b	X m	10~200 ng / 20 m
Nuclease-Free Water	To 20 m

a. The recommended final concentration of primers is When the reaction effect is not good, it can be adjusted in the range of 0.1~ 1 μ M;

B. The recommended template addition amount is 1~ 2 μlIf the template type is undiluted In cDNA stock solution, the amount of template added should not exceed 10% of the total reaction system.Different kinds DNA The number of copies of the target gene contained in the template varies. If necessary, layer dilution may be performed to determine the optimum DNA template addition amount.

3. qPCR Response procedure (can be adjusted according to the model)

two-step method

step	temperature	time
prevariant	95℃	30 sec
denaturation	95℃	10 sec
annealing & extend a	60℃	30 sec
Melting curve b	Use the instrument default acquisition procedure

three-step method

step	temperature	time
prevariant	95

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