peroxidase-conjugated streptavidin
peroxidase-conjugated streptavidin
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brand |
Annoron-Annoron |
item number |
AS000008 |
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Product classification |
alpha-glucosidase |
Research Area |
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productname:peroxidase-conjugated streptavidin Code number:AS000008 Physical state:lyophilized powder Specifications:100 µg Streptavidin concentration:0.7 mg/mL Recommended work concentration: 0.01-0.1 μg/mLforECLWestern blot of substrates 1-2 μg/mLforELISAThe immune tissue/Cytochemical and chromogenic substrates immunoblot Peroxidase:From horseradishrhizomemedium purification buffer:0.01MSodium phosphate,pHvalue is7。4 Stabilizer:15mg/mLBovine Serum Albumin (None)IggNo protease. Preservatives:none Storage andresolution:Store freeze-dried solids in2-8℃Use1.0Ml deionized waterresolution, centrifuge if not clear. Prepare the work dilution on the day of use. The product acts as an undiluted liquid in2-8℃Approximately stable6Week. After resolutionExtended storage:Packed and in-70℃Or freeze below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerin (ACSGrade or higher) to achieve a final concentration of50%And stored in liquid form-20℃under conditions。 Expiration date:fromresolutionFrom date1Years. The valid period may be extended if the test results are appropriate for the intended use. Warning:Before adding to this product, please confirm that the glycerin isACSGrade 1 or higher. Lower grades of glycerol may severely inhibit peroxidase activity.
Test effect comparison:
In multiple batchesDuring the Elisa detection verification process, the trial pack 2 has the same detection signal sensitivity and lower non-specific signal than Jachson. It is recommended thattrial package1The concentration used is1-2Mg/mL, recommendedThe usage concentration of the trial package 2 is 1-2ug/mL, and the customer can optimize the usage concentration of Streptavidin-HRP according to the application scenario in actual testing. |
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