Lactose Bile Salt Fermentation Medium

2026-03-27 10:24:45

  [Number]: HB0110

  [Product name]: Lactose bile salt fermentation medium

  [Specifications]: 250g

  [Purpose]:

　　Lactose Bile Salt Fermentation Medium

　　Name: Lactose Bile Broth

　　Part Number: VIP (HB) 0110

　　Specifications: 250g

　　Uses: Used for the determination of coliform, fecal coliform, and Escherichia coli (GB standard)

Lactose Bile Salt Fermentation MediumComposition (g/L)

　　Peptone 20.0g

　　Bovine bile salt 5.0g

　　Lactose 10.0g

　　Bromocresol Violet 0.01g

　　PH value 7.4 ± 0.2

Lactose Bile Salt Fermentation MediumUsage:

　　Weigh 3.5 grams of this product, heat it and dissolve it in 100ml distilled water, and pack it into inverted fermentation

　　In 20mm x 150mm test tubes, each tube is 10ml, autoclaved at 121 ° C for 15 minutes.

　　Quality control and typical characteristics

　　Cultured at 36 ± 1 ℃ for 24 ± 2 hours, coliform bacteria, Escherichia coli and gram-negative bacteria fermenting lactose produced gas with bubbles in the small inverted tube; other bacteria had no bubbles.

　　Microbial sensitivity test of lactose bile salt fermentation medium:

　　Prepare the medium according to label usage, inoculate the followingquality control strain, placed at 36 ± 1 ° C for aerobic incubation for 24 ± 2 hours.
乳糖胆盐发酵培养基

　　Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services

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