EC broth
[Number]: VIP (HB) 0105
[Product name]: EC broth
[Specifications]: 250g
[Purpose]:
[Product name]: EC broth
[Specifications]: 250g
[Purpose]:
EC broth
Name: E. Coli Broth
Part Number: VIP (HB) 0105
Specifications: 250g
Purpose: used forFecal coliformDetermination of Escherichia coli (GB, SN standards)
EC brothComposition (g/L)
Tryptone 20.0
Lactose 5.0
Sodium chloride 5.0
Dipotassium hydrogen phosphate 4.0
Potassium dihydrogen phosphate 1.5
No. 3 bile salt 1.5
pH value 6.9 ± 0.1 25 ℃
EC brothusage
Weigh 37.0g of this product, heat and stir to dissolve in 1000ml of distilled water, sub-pack into 16mm * 150mm test tubes with inverted fermentation tubes, each tube is 8ml, autoclaved at 121 ° C for 15 minutes, and set aside.
Quality control and typical characteristics
After being incubated at 44.5 ° C for 24-48 hours, fecal coliforms and Escherichia coli produce gas, and there are bubbles in the inverted small fermentation tube.
EC broth microbial sensitivity test:
The media was prepared according to the label usage, inoculated with the following quality control strains, and placed at 44.5 ± 0.2 ° C for aerobic culture for 24-48 hours.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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