mTSB broth
[Number]: VIP (HB) 0148
[Product name]: mTSB broth
[Specifications]: 250g
[Purpose]:
[Product name]: mTSB broth
[Specifications]: 250g
[Purpose]:
mTSB broth
名称：mTSB Broth With Novobiocin
Part Number: VIP (HB) 0148
Specifications: 250g
Purpose: For 0157 selective enrichment (ISO method)
mTSB brothComposition (g/L)
Tryptone 17.0g
Soy peptone 3.0g
Nacl 5.0g
No. 3 bile salt 1.5g
Dipotassium hydrogen phosphate 4.0
Glucose 2.5
Novomycin 0.02
PH value 7.3 ± 0.2 25 ℃
Additives: 6 boxes of VIP (HB) 0106-1 neonatal mycin 4.5mg should be added to each bottle.
Usage: Weigh 33 grams of this product, heat and stir to dissolve in 1000ml distilled water, pack 225ml per bottle, autoclave at 121 ° C for 15 minutes, and add 1 neomycin solution (4.5mg) when cooled to about 50 ° C, mix well, set aside.
Quality control and typical characteristics
10-100 Escherichia coli O157 were inoculated in this medium and cultured at 41.5 ℃ for 18-24h. The growth should be obvious and the solution should be cloudy.
mTSB broth (ISO standard) microbial sensitivity test:
Prepare the medium according to label usage, inoculate the followingquality control strainPlace at 41.5 ± 0.5 ° C for aerobic incubation for 18-24 hours.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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