Escherichia coli coloring medium 9cm
[Number]: VIP (HB) PM7001
[Product name]: Escherichia coli color development medium 9cm
[Specifications]: 9cm * 10 pcs/bag
[Purpose]:
[Product name]: Escherichia coli color development medium 9cm
[Specifications]: 9cm * 10 pcs/bag
[Purpose]:
Escherichia coli coloring medium 9cm (2-8 ℃)
Part Number: VIP (HB) PM7001
Specifications: 9cm * 10 pcs/bag
Uses: For rapid detection of E. coli in food, water, milk, ice cream and meat products.Escherichia coliBlue-green, coliform is colorless, other bacteria are yellow or colorless, Gram-positive bacteria are suppressed.
Escherichia coli coloring medium 9cmComposition (g/L)
Operation steps
1. Prepare the sample solution according to national standards, SN standards, FDA standards or other methods.
2. Take 1ml of the sample solution and add it to the color development medium cooled to 45-50 ° C and mix well or apply it on a plate;
Incubate at 36 ℃ for 18~ 24h, select a plate with 30~ 200 colonies, and count the typical E. coli colonies on the plate. Typical E. coli colonies are blue-green, and other bacteria are colorless or yellow colonies.
Total E. coli = blue-green colony;
4. Suspected Escherichia coli colonies can be inoculated on nutrient agar plates, incubated at 36 ° C for 12-16 hours, and a single colony can be selected for a full set of biochemical tests for Escherichia coli (the company has 4 kinds of biochemical identification tube sets x 4 sets).
Quality Control
1. Appearance
The plate was a transparent colorless solid medium.
2. Microbial testing
Incubate at 36 ° C for 18 to 24 hours:
Limitations of the method
This medium identifies Escherichia coli. In rare cases, false positives may occur, but no false negatives may occur. Sometimes additional tests may be required.
typical features
The typical colony of E. coli is blue-green, coliform is colorless, and other bacteria are colorless or yellow.
Microbial sensitivity test of Escherichia coli coloring medium:
Prepare the medium according to label usage, inoculate the followingquality control strainPlace at 36 ± 1 ° C for aerobic incubation for 18-24 hours.
Note: When calculating the collection rate, TSA agar was used as the control medium.
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