Bacillus cereus coloring medium (
[Number]: VIP (HB) 7021-2
[Product name]: Bacillus cereus color development medium (third generation)
[Specifications]: 1000ml
[Purpose]:
[Product name]: Bacillus cereus color development medium (third generation)
[Specifications]: 1000ml
[Purpose]:
Bacillus cereus coloring medium (third generation)
Part Number: VIP (HB) 7021-2
Specifications: 1000ml
Uses: Used in food, water, dairy and meat productsBacillus cereusRapid detection of. Improved colorants and additives with significantly improved specificity and sensitivity. Bacillus cereus is blue-green with an opaque ring around the colony, other bacteria are blue-green, yellow or colorless, Gram-negative bacteria are suppressed.
Bacillus cereus color development mediumComposition (g/L)
Bacillus cereus color development mediumusage
Weigh 7.95g of the basic medium, add 180ml of distilled water, heat to dissolve and keep stirring; take another 0.6g of Bacillus cereus additive, add 20ml of sterile water, and add some glass balls, keep vibrating for 5-10min to make it emulsify evenly, and autoclave the two at 121 ° C for 15min. When cold to about 45-50 ° C, add the Bacillus cereus additive to the basic medium, gently mix well, and pour into a sterile plate. Make the thickness of the medium about 1-2mm, which is advantageous for observing opaque rings.
Operation steps
1. Prepare sample solution according to GB standard, SN standard, FDA standard or other methods.
The sample solution was incubated at 30 ± 1 ° C for 18-24 hours.
3. Take the enrichment solution and inoculate it on the color medium plate of Bacillus cereus, and culture it at 36 ± 1 ℃ for 18~ 24h. The typical colony of Bacillus cereus is blue-green and there is an opaque ring around the colony. If there is no typical colony in 24 hours, the culture can be extended to 48 hours;
4. Suspected Bacillus cereus can be inoculated on a nutrient agar plate, incubated at 30 ± 1 ° C for 18-24 hours, and a single colony can be selected for a full set of biochemical tests of Bacillus cereus.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is milky white. The prepared plate was opaque and colorless solid medium.
2. Microbial testing
Incubate at 36 ± 1 ° C for 18 to 24 hours:
Limitations of the method
This medium identifies Bacillus cereus, and sometimes false positives may appear, and other supplementary tests are required. Bacillus thuringiensis has the same color as Bacillus cereus on this medium, but it is generally rare in food.
typical features
The typical colony of Bacillus cereus is blue-green with an opaque ring around it
storage
The prepared plate should be used immediately, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, and the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of Bacillus cereus coloring medium (third generation):
Prepare the media according to the label usage, inoculate the following quality control strains, and place them at 36 ± 1 ° C for aerobic culture for 24-48 hours.
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