Candida Color Developing Medium
[Number]: VIP (HB) 7015
[Product name]: Candida coloring medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: Candida coloring medium
[Specifications]: 1000ml
[Purpose]:
Candida Color Developing Medium
Name: Candida Chromogenic Media
Part Number: VIP (HB) 7015
Specifications: 1000ml
Purpose:CandidaColor development medium According to the enzyme substrate method, different Candida are distinguished by color development. After 24-72 hours of culture at 30-35 ° C, Candida albicans appear green, Candida tropical appear blue-gray, Candida smooth appear lavender to purple, Candida krone appear purple-pink, other Candida appear white, and the bacteria are inhibited.
Candida Color Developing MediumComposition (g/L)
Candida Color Developing Mediumusage
Weigh 9.7g of this product (can be expanded or reduced according to the ratio of 4.85g/100ml), add 200ml of distilled water or purified water, heat to dissolve and keep stirring, boil not more than 1 minute. Cool the heated media water bath to 45-50 ° C, shake gently, pour into a sterile flat dish (9cm of 15ml/dish, 7cm of 10ml/dish), after the agar is completely solidified, set aside.
Operation steps
1. Prepare the sample solution according to the Chinese Pharmacopoeia method;
2. Take the sample solution and inoculate it on a suitable medium for cultivation;
3. The suspected colonies were inoculated on the Candida color medium plate and cultured at 30-35 ° C for 24-72 hours. Candida albicans are typically green, tropical Candida is bluish-gray, Candida glossy is lavender to purple, Candida crusadensis is purple-pink, other Candida are white, and the bacteria are inhibited.
4. Further appropriate identification tests should be conducted for suspected Candida albicans colonies.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is light yellow. The prepared plate was a light yellow transparent solid state.
2. Microbial testing
Incubate at 30-35 ° C for 24-72 hours.
Limitations of the method
To identify Candida albicans in this medium, false positives or false negatives may occur in a few cases, and other supplementary tests are required.
typical features
Candida albicans are typically green, tropical Candida is blue-gray, smooth Candida is lavender to purple, Candida krone is purple-pink, and other Candida are white, and the bacteria are suppressed.
storage
The prepared plate should be used immediately, and direct light exposure should be avoided. The dry powder medium should be placed in a dark and dry place at a storage temperature of 2-8 ° C, and should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Candida coloring medium (1000ml) microbial sensitivity test:
Prepare the media according to the label usage, inoculate the following quality control strains, and place them at 25-30 ° C for aerobic culture for 48-72 hours.
Note: SDA agar was used as control medium for collection rate calculation.
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