MEI medium
[Number]: VIP (HB) 8691
[Product name]: MEI medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: MEI medium
[Specifications]: 1000ml
[Purpose]:
MEI medium
Name: MEI Media
Part Number: VIP (HB) 8691
Specifications: 1000ml
Product use: used for one-step filtration method for color detection or counting enterococci in water
Remarks: 4.8% naphthyl ketone acid and 0.4% TTC need to be added together (customers buy their own).
MEI medium usage:
Weigh 72.0g of this product, heat it and dissolve it in 1000ml of distilled water, divide it into triangular bottles, 200ml per bottle, autoclave at 121 ° C for 15 minutes, and cool it to about 50 ° C, add 4.8% naphthyl ketone acid solution 1ml (add a few drops of 0.1N NaOH solution to dissolve) and 0.4% TTC solution 1ml to each bottle, mix well, pour into a sterile flat dish, set aside.
Configuration of 4.8% naphthyl ketone acid:
Weigh 4.8g of naphthyl ketone acid dry powder, add a small amount of purified water and a few drops of 1mo/Lsodium hydroxideDissolve the solution, add purified water to 100ml, filter and sterilize, or autoclave at 121 ° C for 15min.
0.4% TTC configuration:
Weigh 0.4 g TTC and dissolve in 100 ml of distilled water, filter and sterilize.
Characteristics of bacterial colonies on MEI medium:
Enterococcus faecalis was a green colony on MEI medium; Escherichia coli and Staphylococcus aureus were inhibited
MEI medium microbial sensitivity test:
Prepare the media according to the label usage, inoculate the following quality control strains, and place them at 36 ± 1 ° C for aerobic culture for 24-48 hours.
Note: When calculating the collection rate, TSA agar was used as the control medium.
Statement: This reference substance and standard is measured by the central inspection: provide website inquiry and purchase services
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