Enterobacter/coliform coloration culture
[Number]: VIP (HB) 7003-7
[Product name]: Enterobacter/coliform coloring medium (second generation)
[Specifications]: 1000ml
[Purpose]:
[Product name]: Enterobacter/coliform coloring medium (second generation)
[Specifications]: 1000ml
[Purpose]:
Enterobacter/coliform coloring medium (second generation)
Name: E. Coli/Coliform Chromogenic Media
Item number: VIP (HB) 7003-7
Specifications: 1000ml
Purpose: For rapid and accurate simultaneous detectionEscherichia coliAnd coliform, cultured for 24 hours, E. coli appears blue-green-purple, coliform appears red, and Gram-positive bacteria are suppressed.
Enterobacter/coliform coloring mediumComposition (g/L)
This formula can be modified or added with nutrients to achieve the best results.
Enterobacter/coliform coloring mediumusage
Weigh 46.3g of this product and add 1000ml of distilled water, heat to dissolve and keep stirring, boiling for no more than 1 minute. Take 1ml of the prepared sample solution and add it to the center of a 9cm sterile flat dish in diameter, pour it into the medium and cool it to 45-50 ° C for about 15ml, mix well, solidify, and then add a layer of medium to cover.
Or when cooled to 45-50 ° C, pour into a sterile plate, after the agar is completely solidified, place it upside down in an incubator at 37 ° C for 1-2 hours, add 1 ml of sample solution with appropriate dilution, and apply it to the medium. Cultivate at 36 ° C for 18-24 hours.
Operation steps
1. Prepare sample solutions according to national standards, SN standards, FDA standards, or other methods.
2. Take 1 ml of the sample solution and add it to the color development medium cooled to 45-50 ° C to mix well or apply it on a plate.
3. Cultivate at 36 ° C for 18-24 hours, select a plate with 30-200 colonies, and count the typical coliform and Escherichia coli colonies that appear on the plate. Typical colony of Escherichia coli is blue to purple, coliform coliform is pink, and other bacteria are colorless or yellow; total coliform coliform = blue colony + purple colony + pink colony, Escherichia coli = blue colony + purple colony;
4. Suspected Escherichia coli colonies can be inoculated on nutrient agar plates, incubated at 36 ° C for 12-16 hours, and a single colony can be selected for a full set of biochemical tests for Escherichia coli (the company has 4 kinds of biochemical identification tube sets x 4 sets).
Quality Control
1. Appearance
The powder of this dry medium is uniform, has good fluidity, and is milky white to light pink. The prepared plate was a colorless translucent solid medium with a little sediment.
2. Microbial testing
Incubate at 36 ° C for 18 to 24 hours:
Limitations of the method
This medium identifies Escherichia coli/Escherichia coli. In rare cases, false positives may occur, but no false negatives may occur. Sometimes additional tests may be required.
typical features
The typical colony of Escherichia coli is blue to purple, the coliform is pink, and other bacteria are colorless.
storage
The prepared plate should be used immediately, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, and the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of Escherichia coli/coliform coloring medium (second generation):
The media was prepared according to the label usage, inoculated with the following quality control strains, and placed at 36 ± 1 ° C for aerobic culture for 18-24 hours.
Note: When calculating the collection rate, TSA agar was used as the control medium.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
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