Escherichia coli color developing medium
[Number]: VIP (HB) 7001
[Product name]: Escherichia coli color developing medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: Escherichia coli color developing medium
[Specifications]: 1000ml
[Purpose]:
Escherichia coli color developing medium
Name: E. Coli Chromogenic Media
Part Number: VIP (HB) 7001
Specifications: 1000ml
Purpose: Used for rapid color culture of Escherichia coli
Escherichia coli color developing mediumComposition (g/L)
This formula can be modified or added with nutrients to achieve the best results.
Escherichia coli color developing mediumusage
Weigh 50.1g of this product and add 1000ml of distilled water, heat to dissolve and keep stirring, boil not more than 1 minute. Divide into triangular bottles and autoclave at 121 ° C for 15 minutes. Take 1ml of the prepared sample solution and add it to the center of a 9cm sterile plate with a diameter. Pour it into a medium cooled to 45-50 ° C for about 15ml. Mix well. After solidification, culture it inverted at 36 ° C for 18~ 24h. Or when cooled to 45-50 ° C, pour it into a sterile plate. After the agar is completely solidified, place it inverted in an incubator at 37 ° C for 1-2 hours. Add 1ml of the sample solution with appropriate dilution. Coat it on the medium and culture it at 36 ° C for 18~ 24h.
Operation steps
1. Prepare the sample solution according to national standards, SN standards, FDA standards or other methods.
2. Take 1ml of the sample solution and add it to the color development medium cooled to 45-50 ° C and mix well or apply it on a plate;
Incubate at 36 ℃ for 18~ 24h, select a plate with 30~ 200 colonies, and count the typical E. coli colonies on the plate. Typical E. coli colonies are blue-green, and other bacteria are colorless or yellow colonies.
Total E. coli = blue-green colony;
4. Suspected Escherichia coli colonies can be inoculated on nutrient agar plates, incubated at 36 ° C for 12-16 hours, and a single colony can be selected for a full set of biochemical tests for Escherichia coli (the company has 4 kinds of biochemical identification tube sets x 4 sets).
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is milky white. The prepared plate was a transparent colorless solid medium.
2. Microbial testing
Incubate at 36 ° C for 18 to 24 hours:
Limitations of the method
Identification of this mediumEscherichia coliIn a few cases, false positives may occur, but no false negatives may occur. Sometimes additional tests may be necessary.
typical features
The typical colony of E. coli is blue-green, coliform is colorless, and other bacteria are colorless or yellow.
storage
The prepared plate should be used immediately, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, and the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of Escherichia coli coloring medium:
The media was prepared according to the label usage, inoculated with the following quality control strains, and placed at 36 ± 1 ° C for aerobic culture for 18-24 hours. Note: When calculating the collection rate, use TSA agar as the control medium.
Statement: This reference substance and standard substance is provided by [China Inspection and Metrology] for website inquiry and purchase services
Note: Click cas, or search: name, number, cas can display the price.
Previous：Anisin-β-Galactoside Agar (Aliz
