Information Decursinol angelate Decursinol angelate is a cytotoxic and protein kinase C activating agent from the root of Angelica gigas. Targets PKC In vitro Decursinol angelate (DA) blocks PGE2-induced activation of the EP2 receptor signaling pathway, including the activation of PKA and the phosphorylation of CREB. Decursinol angelate also inhibits PGE2-induced expression of cyclooxygenase-2 and the activation of the Ras/Raf/Erk pathway, which activates downstream targets for cell survival. Decursinol angelate greatly reduces the PGE2-induced activation of NF-κB p50 and p65 subunits. It has been reported to suppress the growth of estrogen-stimulated and estrogen-independent growth and survival of breast cancer MCF-7 and MDA-MB-231 cells. Decursinol angelate also suppresses the growth of cancer cells through the inhibition of phosphatidylinositol 3\'-kinase (PI3K)/Akt, extracellular-signal-regulated kinase (Erk) and nuclear factor-κB (NFκB). Decursinol angelate has anti-leukemic activity through the suppression of protein kinase C activation in human leukemia cells. In vivo Decursinol angelate has anti-angiogenic activity both in vitro and in vivo. Cell Research(from reference) Cell lines：MCF-7 cells Concentrations：10 μM, 20 μM, 50 μM Incubation Time：24 hours