Coumberone is a metabolic fluorogenic probe, and isoform-selective substrate for all AKR1C isoforms. Coumberone can be reduced by all four members of the AKR1C family to its fluorescent alcohol coumberol. Coumberone can be used for the research of AKR1C. In Vitro Coumberone (30 μM; 24 hours; HCT116 cells) is an AKR1C3 substrate and demonstrates maximal rates at ~10 μM.\nCoumberone (5 μM; 24 hours; COS-1 cells) can be used for the selective optical readout of each isoform, either AKR1C2 or AKR1C3.\nCoumberone (5 μM; 24 hours; IMR32 cells) enables real-time imaging of AKR1C induction.\nCoumberone (80 hours; IMR-32 cells) metabolism is indeed increased. Coumberone exhibits 10-fold greater catalytic efficiency for AKR1C3 than AKR1C2 in vitro. MCE has not independently confirmed the accuracy of these methods. They are for reference only. ImmunofluorescenceCell Line: IMR32 cells Concentration: 5 μM Incubation Time: 24 hours Result: Enabled real-time imaging of AKR1C induction. Form:Solid