DC0-NH2 is an effector moiety for ADC and a simplified analog of DC1 with better stability. DC0-NH2 is about 1000-fold more cytotoxic than commonly used anticancer agents (ex. Doxorubicin). DC0-NH2 can bind to the minor groove of DNA, followed by alkylation of adenine residues by its propabenzindole (CBI) component In Vitro DC0-NH2 (0-3 nM; 72 hours) is highly potent against Ramos, Namalwa, and HL60/s cells with IC 50 values in the 1 pM to 10 pM range, and has 100 pM range when tested on COLO 205 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Ramos, Namalwa, HL60/s, and COLO 205 Cancer Cells Concentration: 0-3 nM Incubation Time: 72 hours Result: Inhibited Namalwa and HL60/s cells with IC 50 s of 7 and 30 pM, respectively. Form:Solid IC50& Target:Duocarmycins