5'-脱氧腺苷是腺苷的类似物。在S-腺苷甲硫氨酸（SAM）的降解反应中，5'-脱氧腺苷是中间体。5'-脱氧腺苷用于研究磷酸甲基嘧啶合酶，谷氨酸突变酶和5'-甲基硫代腺苷磷酸化酶的运动学。另外，将5'-脱氧腺苷用作质谱标准和胸苷磷酸化酶活性筛选抑制剂。最后，如果进行5'-脱氧腺苷脱氨酶（DadD）分析，则5'-脱氧腺苷起底物的作用。 5'-Deoxyadenosine 是正常受试者尿液中发现的氧化核苷。5'-Deoxyadenosine 具有抗正痘活性。 应用 5′-脱氧腺苷已用于： 作为质谱中的标准品 作为筛选胸苷磷酸化酶活性的抑制剂 作为 5′-脱氧腺苷脱氨酶（DadD）试验的底物 5′-Deoxyadenosine is an analog of adenosin. In the degradation reaction of S-adenosylmethionine (SAM), 5′-Deoxyadenosine is an intermediate. 5′-Deoxyadenosine is used to study the enzyme kinematics of phosphomethylpyrimidine synthase, glutamate mutase, and 5′-methylthioadenosine phosphorylase. In addition, 5′-Deoxyadenosine has been used as a mass spectroscopy standard, and a thymidine phosphorylase activity screening inhibitor. Finally, 5′-Deoxyadenosine functions as a substrate if 5′-Deoxyadenosine deaminase (DadD) assay.