Information JHU-083 JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b + cells and prevents depression-associated behaviors induced by chronic social defeat stress. Solutions are best fresh-prepared. Targets Glutaminase In vitro JHU-083 selectively inhibits T-cell proliferation and decreases T-cell activation, with no effect on DCs. In vivo orally administered JHU-083 significantly decreases the experimental autoimmune encephalomyelitis(EAE) severity in both prevention and treatment paradigms and reverses EAE-induced cognitive impairment. Cell Research(from reference) Cell lines：CD4 + T cells Concentrations：1 μM, 3 μM, 10 μM, 30 μM, or 100 μM Incubation Time：72h