RAS inhibitor Abd-7, a potent RAS-binding compound ( K d =51 nM), is a RAS-effector protein-protein interaction (PPI) inhibitor. RAS inhibitor Abd-7 interacts with RAS inside the cells, prevents RAS-effector interactions and inhibits endogenous RAS-dependent signaling. RAS inhibitor Abd-7 impairs the PPI of various mutant KRAS proteins with PI3K , CRAF and RALGDS as well as NRAS Q61H and HRAS G12V. In Vitro RAS inhibitor Abd-7 (0-20 μM; 24-48 hours) is a cell-potent inhibitor affecting the viability of cancer cell lines in a single digit to low micromolar range. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: DLD-1 cells, HT1080 cells Concentration: 0-20 μM Incubation Time: 24-48 hours Result: The IC 50 of 8 μM in DLD-1 and 10 μM in HT1080 at 72 h (similar values for the IC 50 were found after 48 h). Form:Solid