2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. 它是一种细胞可渗透的非荧光探针。2′,7′-二氯荧光素二乙酸酯是在细胞内脱酯化的并可经氧化转化为具有强荧光的2′,7′-二氯荧光素。 应用包括响应氧化代谢的活性氧的灵敏快速定量；用于吞噬细胞中的氧化产物的微孔板检测，以及定量多孔髓样分化测定。 2′,7′-Dichlorofluorescin Diacetate is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. For maximum solubility in aqueous buffers, DCFH-DA can be directly disolved in 0.1 M Na2CO3 (5 mg/ml) and then immediately diluted with PBS (pH 7.2) to achieve the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1 mg/ml. We do not recommend storing the aqueous solution for more than one day.