PD 404182 is a potent and competitive inhibitor of human dimethylarginine dimethylaminohydrolase 1 (DDAH1) , with an IC 50 of 9 μM. PD 404182 exhibits antiangiogenic and antiviral activity in vitro In Vitro PD 404182 (20 μM) increases asymmetric dimethylarginine (ADMA) approximately 70% in ECs. PD 404182 (50-100 μM; 18 hours) attenuates endothelial tube formation in virto and does not perturb cell membrane integrity or induces cytotoxicity. PD 404182 inhibits HIV-1 in seminal plasma, with an IC 50 of 1 μM. PD 404182 (300 μM;30 minutes) exhibits low toxicity toward several human cell lines , freshly activated PBMCs, primary CD 4+ T lymphocytes, macrophages, and dendritic cells, and lactobacilli found in the normal vaginal flora. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Human dermal microvascular endothelial cells (ECs) Concentration: 10-300 μM Incubation Time: 24 hours Result: Did not induce cytotoxicity. Form:Solid IC50& Target:IC50: 9 μM (DDAH1)