TP0427736是一种有效的ALK5 kinase抑制剂，IC50为2.72 nM，对ALK3的IC50为836 nM。在A549细胞中，它还能抑制TGF-β1诱导的Smad2/3磷酸化，IC50为8.68 nM。 Information TP0427736 is a potent inhibitor ofALK5 kinase activitywith anIC50of 2.72 nM and this effect is 300-fold higher than the inhibitory effect on ALK3 (IC50 = 836 nM for ALK3). It also inhibits Smad2/3 phosphorylation in A549 cells induced by TGF-β1 with an IC50 value of 8.68 nM. Targets ALK5 (Cell-free assay) 2.72 nM In vitro TP0427736 inhibits Smad2/3 phosphorylation in a concentration-dependent manner, with an IC50 value of 8.68 nM. In a cell-based assay of outer root sheath cells, TP0427736 rescued the inhibited proliferation by TGF-β1 and TGF-β2 in a concentration-dependent manner. In vivo TP0427736 decreases shortening of hair follicle length during the transition from the late anagen phase to the catagen phase. Cell Research(from reference) Cell lines：A549 cells Incubation Time：2 h