Listeria coloring medium
[Number]: VIP (HB) 7008
[Product name]: Listeria coloring medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: Listeria coloring medium
[Specifications]: 1000ml
[Purpose]:
Listeria coloring medium
Listera Chromogenic Media
Part Number: VIP (HB) 7008
Specifications: 1000ml
Purpose: Used for color culture of Listeria, can quickly detect single increase in food and medicineListeriaNegative results can be detected within 3 daysListeria monocytogenes is blue with an opaque ring around the colony. Peptone provides a source of nitrogen and amino acids, sodium chloride maintains osmotic pressure, agar is a coagulant, lithium chloride inhibits the growth of gram-negative bacteria, and bacteriostatic agents inhibit the growth of gram-positive bacteria other than Listeria
Listeria coloring medium composition (g/L)
Listeria coloring medium usage
Weigh 14.2g of the basic medium and add 180ml of distilled water, heat and dissolve and stir constantly; take another 1.2g of Listeria additive, add 20ml of sterile water, and add some glass balls, keep vibrating for 1-2min to mix evenly, and autoclave the two at 121 ° C for 15 min. When cold to about 50 ° C, add the Listeria additive to the basic medium, and add 1 piece of the redissolved Listeria bacteriostatic agent, mix well, and pour into a sterile plate. Make the thickness of the medium about 2-3mm, so that it is advantageous to observe the opaque ring.
Operation steps
Before use, place the prepared medium at room temperature for 10 to 20 minutes.
1. Prepare sample solution according to SN standard, GB standard, FDA standard, ISO standard or other standards.
2. Add the sample solutionLee's bacterial broth(LB1, LB2) to increase bacteria; or mix the sample solution and the Half-Fraser bacterial solution in a ratio of 1:10 and incubate at 30 ° C for 24 hours; or other bacterial solutions to increase bacteria.
3. Take a ring of bacterial solution and inoculate it on Listeria coloring medium, and incubate it at 36 ° C ± 1 ° C for 24-48 hours.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is light yellow. The prepared plate was opaque milky white medium.
2. Microbial testing
typical features
Listeria monocytogenes were cultured at 36 ° C ± 1 ° C for 24-28 hours, and blue colonies appeared on the plate with an opaque ring around the colony. Listeria sheep were cultured at 36 ° C ± 1 ° C for 48 hours, and blue colonies appeared on the plate with an opaque ring around the colony. Listeria stella was cultured at 36 ° C ± 1 ° C for 24-48 hours, and blue colonies appeared on the plate with no opaque ring around the colony. Listeria sheep is very rare in food testing.
storage
The prepared solid plate should be stored at 2-8 ° C, avoiding direct light exposure. The drying medium should be placed in a dark and dry place, and the storage temperature should be 2-8 ° C.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
references
1、VAN NETTEN, P., PERALES, J., VAN DE MOOSDIJK, A., CURTIS, G.D.W., a. MOSSEL, D.A.A.: Liquid and solid selective differential media for the detection and enumeration of Listeria monocytogenes. Int. Food Microbiol., 8; 299-316 (1989).
2、HAMMER, G., HAHN, G., KIRCHHOFF, H., a. HEESCHEN, W.: Comparison of the suitability of Oxford and PALCAM medium for the isolation oflistaria moNocytogenes from soft cheese. German Dairy Industry, 41; 334-336 (1990).
Microbial sensitivity test of Listeria coloring medium 1000ml:
Prepare the medium according to the label usage, inoculate the following quality control strains, and place them at 37 ° C for aerobic culture for 24-48 hours.
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