Salmonella Color Developing Medium
[Number]: VIP (HB) 7007
[Product name]: Salmonella color medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: Salmonella color medium
[Specifications]: 1000ml
[Purpose]:
Salmonella Color Developing Medium
Name: Salmoin the Chromogenic Medium
Part Number: VIP (HB) 7007
Specifications: 1000ml
Uses: For rapid detection of Salmonella in food, water, milk, ice cream and meat products. Except for Salmonella typhi (colorless on the medium), which cannot be directly identified, other Salmonella are bright red, Escherichia coli and Escherichia coli are blue, Citrobacter is purple, and other bacteria are yellow or colorless. In food contamination, the main Salmonella is other Salmonella other than Salmonella typhi. The specificity of this medium is very high, up to 98%. It is an ideal medium for rapid detection.
Salmonella Color Developing MediumComposition (g/L)
usage
Weigh 7.26g of this product, add 200ml of distilled water, add 3ml of additives, heat to boil to dissolve and keep stirring, heat for 10-20 minutes. When cooled to 45-50 ° C, pour into a sterile plate.
Attention: The prepared medium must be kept dry on the surface and can be placed upside down in an incubator at 37 ° C for 1-2 hours.
Operation steps
1. Prepare the sample solution according to national standards, SN standards, FDA standards or other methods.
2. Take 1 ml of sample solution and add it to 9 ml of RVS broth or other selective bacteria-increasing broth, and incubate it at 36 ° C 1 ° C for 242 hours;
3. Use a 3mm inoculation ring to take 1 ring of bacterial enhancement liquid, scribe and inoculate it on the Salmonella color-developing medium, and make 2 plates;
4. Cultured at 36 ℃ for 24~ 28h, Salmonella (except Salmonella typhi) was bright red, E. coli and E. coli were blue, Citrobacter was purple, and other bacteria were yellow or colorless;
5. Suspected colonies can be inoculated on nutrient agar plates, incubated at 36 ° C for 12-16 hours, and single colonies can be selected for a full set of biochemical tests and serological tests for Salmonella (the company has a biochemical identification tube set).
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is light red. The prepared plate was light red solid medium.
2. Microbial testing
Incubate at 36 ° C for 24 to 28 hours:
Limitations of the method
This medium cannot directly identify Salmonella typhi. The specificity of other Salmonella can reach 98%, and the accuracy is very high. Sometimes additional tests may be required.
typical features
Typical Salmonella appears bright red, E. coli appears blue-green, Citrobacter appears purple, and other bacteria appear yellow or colorless.
storage
The prepared plate can be stored for 2-5 days, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Statement: This reference substance and standard is measured by the central inspection: provide website inquiry and purchase services
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