O157 color development medium
[Number]: VIP (HB) 7006
[Product name]: O157 color development medium
[Specifications]: 1000ml
[Purpose]:
[Product name]: O157 color development medium
[Specifications]: 1000ml
[Purpose]:
O157 color development medium
Name: O157 chromogenic medium
Part Number: VIP (HB) 7006
Specifications: 1000ml
Uses: Used for rapid detection of O157: H7 bacteria in food and patient fecal samples. O157: H7 bacteria are bright red, light red or red, there is no blue halo around the colony, Escherichia coli and coliform bacteria are dark blue, blue or purple, there is a blue halo around the colony; other bacteria are blue, yellow or colorless. This medium has high specificity and can overcome the false positive and false negative results caused by SMAC medium. It is an ideal medium for rapid detection.
O157 color development mediumComposition (g/L)
usage
Weigh 17.2g of this product and add 200ml of distilled water, heat to boil to dissolve and keep stirring, heat for about 2 minutes. When cooled to 45-50 ° C, add a CT additive and mix well, pour into a sterile plate.
Attention: The prepared medium must be kept dry on the surface and can be placed upside down in a 36 ° C incubator for 1-2 hours.
Operation steps
1. Prepare the sample solution according to national standards, SN standards, FDA standards or other methods.
2. Take 25 grams of sample solution and add it to 225 ml of mEC broth or mTSB broth, and incubate it at 36 ° C for 18-24 hours;
3. Use a 3mm inoculation ring to take 1 ring of bacterial enhancement liquid, scribe and inoculate it on the color-developing medium of O157 bacteria, and make 2 plates;
4. Cultured at 36 ° C for 18-24h, O157: H7 bacteria showed bright red, reddish or red, and there was no blue halo around the colony; Escherichia coli and Escherichia coli showed dark blue, blue or purple, and there was a blue halo around the colony; other bacteria showed blue, yellow or colorless;
5. Typical colonies can be tested for O157: H7 bacteria serology and a full set of biochemical tests.
Note: Results must be interpreted within 24 hours of incubation at 36 ° C to avoid changes in colony color.
Quality Control
1. Appearance
The powder of this drying medium is uniform, has good fluidity, and is gray-white. The prepared plate was blue solid medium.
2. Microbial testing
Incubate at 36 ° C for 18-24 hours:
Limitations of the method
Typical E. coli O157: H7 on this medium must be tested serologically or biochemically.
typical features
Typical Escherichia coli O157: H7 bacteria appear bright red, reddish or red with no blue halo around the colony; Escherichia coli and Escherichia coli appear blue or purple with blue halo around the colony; other bacteria appear blue, yellow or colorless;
storage
The prepared plate can be stored for 2-5 days, and direct light exposure should be avoided. The drying medium should be placed in a dark and dry place, the storage temperature should be 2-8 ° C, and it should be stored away from light.
failure
Dry media should not be used beyond shelf life, clumping, or color changes.
Attention
This medium is for laboratory use only.
Microbial sensitivity test of O157 color development medium:
The media was prepared according to the label usage, inoculated with the following quality control strains, and placed at 36 ± 1 ° C for aerobic culture for 18-24 hours.
Statement: This reference substance and standard is measured by the central inspection: provide website inquiry and purchase services
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